Journal: ACS Materials Au

Article Title: 3D-Printed Biohybrid PE/PCL Biphasic Osteochondral Plug for Knee Cartilage Repair

doi: 10.1021/acsmaterialsau.5c00193

Figure Lengend Snippet: CHON-001 ATCC test on PCL–RGD and PCL–HA samples compounded in PCL (Resomer C212, Evonik Industries AG). Cell proliferation assay was performed in human chondrocytes seeded onto PCL-based materials. Fold change in chondrocyte numbers was assessed in each biomaterial compared to control (PCL) by Kruskal wallis test whereby * is p value <0.05. Representative data with 4 technical replicates per group.

Article Snippet: Human chondrocytes (CHON-001) were purchased from ATCC (Cat No: CRL-2846) and maintained in complete media (Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum and 0.1 mg/mL G418) and incubated at 37 °C with 95% Air and 5% CO 2 as per ATCC recommendation.

Techniques: Proliferation Assay, Control

Journal: ACS Materials Au

Article Title: 3D-Printed Biohybrid PE/PCL Biphasic Osteochondral Plug for Knee Cartilage Repair

doi: 10.1021/acsmaterialsau.5c00193

Figure Lengend Snippet: Porcine knee joint at 24 weeks with OC plug implanted (a), extracted sample with 68% closure measured using Fiji software (b), porcine knee joint with microfracture (c). H&E staining images for corresponding knee joints where (d) cartilage with significant viable chondrocytes are observed in sample with plug embedded (white space is the OCP) and (e) Little cartilage growth in sample with microfracture.

Article Snippet: Human chondrocytes (CHON-001) were purchased from ATCC (Cat No: CRL-2846) and maintained in complete media (Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum and 0.1 mg/mL G418) and incubated at 37 °C with 95% Air and 5% CO 2 as per ATCC recommendation.

Techniques: Software, Staining

Journal: BMC Musculoskeletal Disorders

Article Title: ARG098, a novel anti-human Fas antibody, suppresses synovial hyperplasia and prevents cartilage destruction in a severe combined immunodeficient-HuRAg mouse model

doi: 10.1186/1471-2474-11-221

Figure Lengend Snippet: ARG098 cytotoxicity to human chondrocytes . (A) ARG098 binding activity to human chondrocytes was assessed by flow cytometory. The white area is stained with human IgM and the black area is stained with ARG098. (B) RA synoviocytes pretreated with anti-Fas neutralizing antibody clone SM1/23. (C) Representative cell viability of human chondrocytes following ARG098 treatment (Experiment 1: from 0.1 to 1000 ng/mL, Experiment 2: from 1 to 100 μg/mL) versus untreated condition, as measured by the WST assay. Black circles and black triangles, ARG098; white circles and white triangles, human IgM. Each point represents mean ± SEM (n = 5).

Article Snippet: The sources of the other materials used in this study are as follows: human IgM was obtained from ICN Biomedicals Inc. (Aliso Viejo, CA, USA), chondrocyte basal medium supplemented with chondrocyte growth supplement was obtained from Cell Applications, Inc. (San Diego, CA, USA), and the neutralizing antibody anti-human APO-1/Fas (SM1/23) was obtained from Bender Medsystems GmbH (Vienna, Austria).

Techniques: Binding Assay, Activity Assay, Staining, WST Assay